AskDefine | Define blotting

Dictionary Definition

blot

Noun

1 a blemish made by dirt; "he had a smudge on his cheek" [syn: smudge, spot, daub, smear, smirch, slur]
2 an act that brings discredit to the person who does it; "he made a huge blot on his copybook" [syn: smear, smirch, spot, stain]

Verb

1 dry (ink) with blotting paper
2 make a spot or mark onto; "The wine spotted the tablecloth" [syn: spot, fleck, blob] [also: blotting, blotted]blotting See blot

User Contributed Dictionary

English

Verb

blotting
  1. present participle of blot

Extensive Definition

In molecular biology and genetics, a blot is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulose PVDF or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by one or more different methods:
  • colorant staining (for example, silver staining of proteins)
  • autoradiographic visualization of radioactive labelled molecules (performed before the blot)
  • specific labelling of some proteins or nucleic acids. It is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme joined to them. After proper washing, this enzymatic activity (and so, the molecules we search in the blot) is visualized by incubation with proper reactive, rendering either a colored deposit on the blot or a chemiluminiscent reaction which is registered by photographic film.
blotting in German: Blotting
blotting in Persian: بلاتینگ
blotting in French: Blot (biologie)
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